Plant Pest Diagnostics Center
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Dr. Dan C. OpgenorthSenior Plant Pathologist Plant Pest Diagnostics Branch, California Department of Food & Agriculture
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Curriculum vitae
Education
- Doctor of Philosophy (Plant Pathology) - University of California, Riverside, 1980: GPA 3.7.
- Master of Science (Plant Pathology) - University of California, Riverside, 1973: GPA 3.7.
- Bachelor of Science with Honors (Biochemistry) - University of Wisconsin, Madison, 1969: GPA 3.5.
- Certified Plant Pathologist #24946 and Member of the Certification Board American Society of Agronomy
Experience:
- Currently working with U.C. Farm Advisors and Researchers at Parlier concerning the detection of Corn Stunt Spiroplasma using ELISA and PCR techniques on Plant and Insect samples.
- Development of Real-Time PCR with a USDA cooperator, for rapid detection of significant regulatory pathogens at Federal Port Facilities in California (Citrus Canker).
- Participation in NATO Exchange program with Macedonia to train foreign scientists.
- Maintenance of the Bacterial Culture Collection acquired from U. C. Berkeley Department of Plant Pathology.
- Development of Rep-PCR for identification of Plant Pathogenic Bacteria and use of PCR for the detection of Phytoplasma.
- Production of Monoclonal Antibodies for the detection of Prunus Necrotic Ringspot Virus. (Patents with U. C. Berkeley)
- Establishment of an ELISA Laboratory at CDFA to test for regulatory plant viruses; including contract work with biotechnology companies and grant work on stone fruit virus diseases.
- Chairman of the California Plant Disease Conference: responsible for the organization of seminars concerning Pesticide Use, Grape Pathology, Seed Pathology and Stone Fruit Virus Diseases.
- Responsible for all laboratory work concerning the detection of genetically altered ice minus bacteria (Frost Ban) released into the environment on strawberry test plots in California.
- Negotiated contractual arrangements with scientists using specific gene probes to positively identify the genetically altered bacteria.
- Graduate Research Assistant conducting thesis research on Fusarium Yellows of Celery. (Major Professor: Dr. Robert Endo)
- USDA Extension Specialist operating the Plant Disease Diagnostic Clinic at the Univ. of Wisconsin Madison. Supervisor: Dr. Gayle Worf
- Research Associate at Cornell Univ. Conducting field work on the epidemiology and control of Fire Blight. Supervisor Dr. Steven Beer.
- Ph.D. Thesis: Sources of Resistance to Fusarium Yellows of Celery; studies of chlamydospore formation and germination; effects of inoculum density and pH on disease development.
Teaching
- Involved in the training and supervision of all Research Associates and Seasonal Employees working in the CDFA Bacteriology Laboratory.
- Teaching Assistant in a basic Plant Pathology course for pest management majors under supervision of Professor Donald C. Erwin.
Interests:
- Diagnostic Plant Pathology; Antique Auto Restoration; Motorcycling; Scouting; Little League; bicycling, skiing, camping, hiking etc. Member American Phytopathological Society and Buick Club of America
Memberships
- American Phytopathological Society
- Buick Club of America.
Military:
- U. S. Army, 1970-72. Stationed at Letterman General Hospital San Francisco in the pathology section doing directed research using electron microscopy.
References:
- Dr. Conrad Krass; Principal Staff Pathologist at CDFA (916) 654-1009
- Mr. William Callison Branch Chief CDFA (916) 262-1100
- Dr. Bruce Kirkpatric U. C. Davis Plant Path (510) 445-6214
- Dr. Charles Summers Kearney Agricultural Center (559) 646- 6500.
Literature Published
- Worf, G. L. and D. C. Opgenorth. 1974. Chemical control of Snow Mold (Typhula spp.) on turf. American Phytopathological Society Fungicide-Nematicide .Results for 1974.
- Beer, S. v. and D. C. Opgenorth. 1976. Erwinia amylovora on flreblight canker surfaces and blossoms in relation to disease occurrence. Phytopathology 66: 311-317.
- Opgenorth, D. C. and R. M. Endo. 1979. Sources of resistance to Fusarium Yellows of Celery. Plant Disease Reporter 63: 165-169.
- Opgenorth, D. C. and R. M. Endo. 1981. Competitive Saprophytic Ability (CSA) of Fusarium oxysporium f.sp. apii. Phytopathology 71: 246
- Lai, M., D. Opgenorth and J. B. White. 1982. Occurrence of Pseudomonas corrugata on Tomato in California. Plant Disease 67: 110.112.
- Opgenorth, D.C. 1982. Storage Rot of California Grown Kiwifruit. Plant Disease 67: 382-383.
- Michailides, T. J., J. M. Ogawa and D. C. Opgenorth. 1987. Shift of Monilinia spp. and distribution of isolates sensitive and resistant to Benomyl in California Prune and Apricot orchards. Plant Disease 71: 893-896.
- Braun, A. L. and D. C. Opgenorth: 1987. Comparative detectability of potato leafroll virus, potato virus X and potato virus S using enzyme-linked immunosorbant assy (ELISA) and serologically specific electron microscopy (SSEM). American Potato Journal 64: 205-209
- Opgenorth, D. C. and J. Smith. 1989. ELISA testing program for stone fruit viruses initiated in California. Phytopathology 1989
- Opgenorth, D. C.. and I. Smith. 1989. Production ofmonoc1ona1 antibodies to strains of Prunus Necrotic Ringspot Virus. Phytopathology 1989.
- Opgenorth, D. C., I. White and P. Gunnell. 1989. Strawberry Anthracnose in California. California Plant Pest and Disease Report (Sept-Dec) 1989.
- Opgenorth, D. C. and J.White. 1991. Hainesia Leaf Spot on Strawberry. California Plant Pest and Disease Report (Ian-May) 1991.
- Opgenorth, D. C. and J. White. 1991. Freeway Daisy (Osteospermum fructicosum) as host for Lettuce Mosaic Virus. Plant Disease 75: 751.
- Opgenorth, D. C. Alexander Karu and J. Smith. 1991. Monoclonal Antibodies for identification of Prunus Necrotic Ringspot Virus and Related Stone Fruit Viruses (Licensing Disclosure for Patent); U. C. Berkeley Office of Technology Licensing Invention and Technology. 1991.
- Opgenorth, D. C.. and J. White. 1992. Pear-Juniper Rust. Ca. Plant Pest and Dis. Report. Opgenorth, D. C. 1993. Olive Knot and Oleander Gall. Ca. Plant Pest and Dis. Report.
- Opgenorth, D. C. Sauber, and I. King 1993. First Report of Westeria Gall in California. California Plant Pest and Disease Report (Oct-Dec)
- Opgenorth, D. C., Connolly, K. and L. Braun. 1994. Bacterial Leaf Spot Unique to Manzanita sp. (Arctostaphylous densiflorus var. Howard McMinn). Ca. Plant Pest and Dis. Report.
- Opgenorth, D. C. 1994. Potato (common) Scab. Ca. Plant Pest and Dis. Report.
- Opgenorth, D. C. 1994. Drippy Nut Disease. Ca. Plant Pest and Dis. Report.
- Opgenorth, D. C., V Takikawa, Y ., M. Hendson and E. Clark. 1994. First Report of Bacterial Gall of Westeria sinensis caused by Erwinia herbicola pv. millettiae in California. PlantDisease 78: 1217.
- Opgenorth, D. C. 1995. Bacterial Fruit Blotch of Watermelon. Ca Plant Pest and ills. Report.
- Opgenorth, D. C. and C. Smart 1996. Identification of Xanthomonas fragariae Field Isolates by Rep-PCR Genomic Fingerprinting. Plant Disease 80: 868-873.
- Opgenorth, D. C. 1998. Bacterial Canker of Tomato. Ca. Plant Pest and Dis. Report.
- Louws, F. I., C. M. .Medina-Mora, J. Bell, C. D. Smart, Opgenorth, D. C., C. A. Ishimaru, M. K. Hausbeck, F. J. de Bruijn and D. W. Fulbright. 1998. Rep-PCR Mediated Genetic Fingerprinting is a Highly Discriminatory Method to Differentiate Subspecies of Clavibacter michiganensis. Phytopathology 88: 862-868.
- Schaad, N.W., D. C. Opgenorth and P. Gaush. 2002 Real-Time Polymerase Chain Reaction for one-Hour On-Site Diagnosis of Pierces Disease of Grape in Early Season Asymptomatic Vines. Phytopathology 92: 721-727.
- Schaad, N.W., D. Opgenorth. M. D. Petrillo, M. A. Abdelshife and T. Tedla. 2003. Evaluation of a On-Site, One –houe Real Time PCR Assay for Detecting the Citrus Canker Bacterium in Plant Samples at Port Facilities. APS Poster at Annual Meetings 2003.
- Summers, C. A., A. S. Newton and D. C. Opgenorth. 2004. Overwintering of Corn Leafhopper, Dalbulus maidis (Homoptera: Cicadellidae), and Spiroplasma kunkelii (Mycoplasmatales: Spiroplasmataceae) in Californias San Joaquin Valley. Journal of Environmental Entomology 33: No. 6 pp1644-1651.
- Wei. W., D. C. Opgenorth, R. E. Davis., and Y. Zaho. 2006. Characterization of a Novel Adhesion Gene and Design of a Real-Time Polymerase Chain Reaction for Rapid, Sensitive and Specific Detection of Spiroplasma kunkelii Phytopathology (in press)



